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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
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008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
23/06/2021 |
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Tipo de producción científica : |
Documentos |
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Autor : |
FASSIO, A.; GARCÍA, A.; GIMÉNEZ, A. |
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Afiliación : |
ALBERTO SANTIAGO FASSIO ARAUJO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIANA GARCIA LAMOTHE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AGUSTIN EDUARDO GIMÉNEZ FUREST, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Respuesta a fertilización nitrogenada en girasol. |
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Fecha de publicación : |
1995 |
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Fuente / Imprenta : |
ln: INIA. Programa Cultivos de Verano, Programa Evaluación de Cultivares. Día de campo. Cultivos de verano, 1995, La Estanzuela. Colonia (UY): INIA La Estanzuela, 1995. |
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Páginas : |
p. 3-4 |
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Serie : |
(INIA Serie Actividades de Difusión ; 42) |
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Idioma : |
Español |
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Thesagro : |
ABONOS NITROGENADOS; GIRASOL; HELIANTHUS ANNUUS; RESPUESTA DE LA PLANTA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7401/1/UY.INIA.SAD.1995.n.42.pdf;campo#page=7
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Marc : |
LEADER 00696naa a2200205 a 4500
001 1047128
005 2021-06-23
008 1995 bl uuuu u00u1 u #d
100 1 $aFASSIO, A.
245 $aRespuesta a fertilización nitrogenada en girasol.
260 $c1995
300 $ap. 3-4
490 $a(INIA Serie Actividades de Difusión ; 42)
650 $aABONOS NITROGENADOS
650 $aGIRASOL
650 $aHELIANTHUS ANNUUS
650 $aRESPUESTA DE LA PLANTA
700 1 $aGARCÍA, A.
700 1 $aGIMÉNEZ, A.
773 $tln: INIA. Programa Cultivos de Verano, Programa Evaluación de Cultivares. Día de campo. Cultivos de verano, 1995, La Estanzuela. Colonia (UY): INIA La Estanzuela, 1995.
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